Depth-resolved measurement of the dermal matrix composition by multiphoton laser tomography.

BACKGROUND: In the last years, multi photon laser tomography (MLT) has emerged as a promising tool for non-invasive diagnostics in dermatology and other medical specialties. The present work is dedicated to the question to what degree the measurement depth and the thickness of the epidermis influence the evaluation of dermal matrix composition and if recommendations for future measurement procedures can be given. METHODS: In a study group of 30 healthy volunteers aged 21-82 years multiphoton depth-resolved measurements of autofluorescence and second harmonics have been performed in order to evaluate the dermal matrix composition. RESULTS: Characteristic intensity curves depending on the penetration depth were derived and differences between age groups were found. CONCLUSION: With the present work we provide evidence for the accuracy of the measurement of dermal matrix composition by MLT and give detailed advice for the measurement procedure. Furthermore, we propose the use of depth-dependent emission intensity curves for monitoring of anti-aging treatment.