Effects of Pulsed Electromagnetic Fields on Breast Cancer Cell Line MCF 7 Using Absorption Spectroscopy.

Author: Alcantara DZ1, Soliman IJS1, Pobre RF1, Naguib RNG2,3
1Optics and Instrumentation Physics Laboratory, Physics Department, De La Salle University, Manila, Philippines.
2Faculty of Science, Liverpool Hope University, Liverpool, U.K. naguibr@hope.ac.uk r.naguib@biocoreinternational.com.
3BIOCORE Research & Consultancy International, Liverpool, U.K.
Conference/Journal: Anticancer Res.
Date published: 2017 Jul
Other: Volume ID: 37 , Issue ID: 7 , Pages: 3453-3459 , Word Count: 217

We present an analysis of the effects of pulsed electromagnetic fields (PEMF) with 3.3 MHz carrier frequency and modulated by audio resonant frequencies on the MCF-7 breast cancer cell line in vitro using absorption spectroscopy. This involves a fluorescence dye called PrestoBlue™ Cell Viability Reagent and a spectrophotometry to test the viability of MCF-7 breast cancer cells under different PEMF treatment conditions in terms of the cell absorption values. The DNA molecule of the MCF-7 breast cancer cells has an electric dipole property that renders it sensitive and reactive to applied electromagnetic fields. Resonant frequencies derived from four genes mutated in MCF-7 breast cancer cells [rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR), peroxisome proliferator-activated receptor (PPARG), Nijmegen breakage syndrome 1 (NBN) and checkpoint kinase 2 (CHEK2)] were applied in generating square pulsed electromagnetic waves. Effects were monitored through measurement of absorption of the samples with PrestoBlue™, and the significance of the treatment was determined using the t-test. There was a significant effect on MCF-7 cells after treatment with PEMF at the resonant frequencies of the following genes for specific durations of exposure: RICTOR for 10 min, PPARG for 10 min, NBN for 15 min, and CHEK2 for 5 min.

Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

KEYWORDS: Breast cancer; PrestoBlueT™; gene mutation; pulsed electromagnetic fields; spectroscopy

PMID: 28668834