Studies of the 'external qi' effect on the ConA mediated agglutination reaction of human pulmonary adenocarcinoma cell SPC-A-1

Interrecognition and contact inhibition are the main characteristics of cultured animal cell, which can usually appear agglutination reaction mediated by some plant lectin, such as ConA.. It has been proved that there is close relationship between the agglutination and the glycoproteins on cell membrane, the sort and the distribution of glycoproteins as well as their interaction with the relative receptors can all influence the agglutination reaction. Generally speaking, normal cells express less degree of agglutination than tumour cells. According to this principle, we compared the difference of control cell SPC-A-1 from 'external qi'treated cell in their agglutination, and preliminarily studied the mechanism from the change of glycoprotein on cell membrane.

From many times of repeated experiments, we found 'external qi' treatment can surely reduce the extent of agglutination, and this effect became more and more obvious with increasing times of treatment. Meanwhile, the kinds of glycoprotein on cell membrane were also changed displayed by SDS-PAGE with glycoprotein specific Schiff's staining method, some bands disappeared and others emerged which may imply their relativity with cell recognition. When the FITC labeled ConA was used as a fluorescent probe to detect glycoprotein and their 'receptors' change in situ with FCM technique, this result was further evidenced that the distribution of some relative molecules have also varied. So this experiment provided us another evidence for the cancer cell reversion by qigong treatment at the level of cell physiology.

Key Words: human pulmonary adenosarcomas cell, external qi, qigong, ConA Mediated Agglutination Reaction