Osteogenic differentiation of adipose-derived stem cells prompted by low-intensity pulsed ultrasound.
Author: Yue Y, Yang X, Wei X, Chen J, Fu N, Fu Y, Ba K, Li G, Yao Y, Liang C, Zhang J, Cai X, Wang M.
Affiliation:
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Conference/Journal: Cell Prolif.
Date published: 2013 Jun
Other:
Volume ID: 46 , Issue ID: 3 , Pages: 320-7 , Special Notes: doi: 10.1111/cpr.12035. , Word Count: 167
OBJECTIVES:
Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) regulated by LIPUS, and also in a preliminarily manner, we have discussed diverse effects of different duty ratio parameters.
MATERIALS AND METHODS:
Mouse adipose stem cells were isolated and osteogenically induced. Then they were treated with LIPUS for 10 min/day for 3 days, 5 days and 7 days, respectively. Finally, effects of LIPUS on osteogenic differentiation of the ASCs were analysed by real-time PCR, western blotting and immunofluorescence.
RESULTS:
Our data indicated that LIPUS promoted mRNA levels of runt-related transcription factor 2, osteopontin and osterix in the presence of osteo-induction medium; moreover, protein levels of runt-related transcription factor 2 and osteopontin were upregulated.
CONCLUSIONS:
We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.
© 2013 Blackwell Publishing Ltd.
PMID: 23692090 [PubMed - in process]
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