Author: Chen SH, Sun H, Xu H, Zhang YM, Gao Y, Li S.
Chinese Academy of Medical Science, Peking Union Medical College, Department of Chinese Medicine, Peking Union Medical College Hospital, Beijing 100730, China.
Conference/Journal: Zhen Ci Yan Jiu.
Date published: 2012 Dec
Other: Volume ID: 37 , Issue ID: 6 , Pages: 470-5 , Special Notes: [Article in Chinese] , Word Count: 284
To observe the effects of acupuncture of "Baihui" (GV 20) and "Zusanli" (ST 36) on the expression of interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha proteins in the bilateral cerebral tissue in cerebral ischemia reperfusion injury (CI/RI) rats, so as to explore its mechanism underlying improving CI.
Seventy-eight SD rats were randomized into sham-operation (control) group (n = 6), model group (n = 36) and acupuncture group (n = 36), and the latter two groups were further divided into 12, 24, 48, 72, 96 and 144 h subgroups (n = 6 in each subgroup) according to the reperfusion time. Acupuncture stimulation was applied to GV 20 and left ST 36 for 20 min at the required time-points after successful operation, once a day. The expression of IL-1beta and TNF-alpha in bilateral brain tissue was examined by immunohistochemistry.
Immunohistochemical staining showed that the expression of cerebral IL-1beta protein in cerebral ischemia regions exhibited a double-peak, and the peak time in the model group was at 48 h and 96 h, while the expression of TNF-alpha protein in cerebral ischemia regions exhibited a single-peak, and the peak time in both model and acupuncture groups was at 72 h. In comparison with the normal group and sham group, cerebral IL-1beta and TNF-alpha protein expression levels of both ischemic and non-ischemic regions were increased significantly in the model group (P < 0.05). Compared to the model group, the expression levels of cerebral IL-1beta and TNF-alpha of both ischemic and non-ischemic regions in the acupuncture group at most of the time-points after CI/RI were decreased obviously (P < 0.05).
Acupuncture can effectively suppress the expression of IL-1beta and TNF-alpha proteins in bilateral ischemic and non-ischemic cerebral regions in CI/RI rats,suggesting an anti-inflammatory effect of acupuncture intervention.