Curative effect of emitted qi on mice with MO4 tumors

EXPERIMENTS

Experiment 1

Seventeen male mice were injected intraperitoneally with 1X10exp5 MO4 cells in 0.2 ml serum free culture medium. The mice were divided randomly into two groups (the treating group and control group). Since the second day after implantation of M04 cells, the mice of the treating group were treated every day for 30 minutes by the emitted qi given out through the hands of qigong doctor Shen Hongxun. The distance between the mice and the hand was 15 to 20 cm.

The control group was not given any treatment. The mice of the two groups were kept on the same standard pellet diet and tap water ad libitum. The time of death of the mice was noted down and all the dead mice were autopsied and the volume of the tumor was measured.

Experiment 2

Seventeen female mice were injected intraperitoneally with 1X10exp5 M04 cells in 0. 2 ml serum culture medium. The mice were divided randomly into the treating group and control group. On the seventh day after implantation of MO, cells the treatment was started and the curative method and examination were the same as in the 1st experiment.

Experiment 3

Twenty female mice were injected subcutaneously into the right axilla with 1X10exp5 MO4 cells in 0. 2 ml serum-free culture medium and then the mice were divided randomly into the treating group and control group. On the seventh day after implantation of MO4 cells, the tumor could be touched and the treatment was started. The curative method and the examination were the same as in the 1st experiment.

RESULTS

In experiment 1 and 2 all mice died within 28 days and all dead mice showed intra-abdominal tumor and hemorrhagic ascites. Most of the tumor nodes and the hemorrhagic as cites were collected and their volume was measured individually.

In experiment 3 the mice of the control group died within 34 days, but the mice of the treating group lived longer. The tumor localized under the skin and some tumor had invaded into the pleural cavity. The tumor volume was measured individually according to the formula: v = a x bexp2 x O.4exp8. V is the tumor [volume], a is the long diameter of the tumor, b is the perpendicular short diameter of the tumor.

Through statistical analysis we found that in experiment 1 and 2 the volume of the intraabdominal tumors had a significant difference between the treating group and the control group (in experiment l, p=O.0024, in experiment 2, p<0.000)but the survival period and the volumes of the bloody ascites did not have a statistical difference between the treating group and the control group.

In experiment 3 by statistical comparison, we found that the survival period and the average tumor volume per day had significant difference between the treating group and the control group (P survival time=O. 02, p tumor volume=O. 012), but the end volume of the tumor did not have any significant difference 0.105.