The Use of Natural Agents to Counteract Telomere Shortening: Effects of a Multi-Component Extract of <i>Astragalus mongholicus</i> Bunge and Danazol.

Author: Guinobert I1, Blondeau C1, Colicchio B2, Oudrhiri N3, Dieterlen A2, Jeandidier E4, Deschenes G5, Bardot V1, Cotte C1, Ripoche I6, Carde P7, Berthomier L6, M'Kacher R8
Author Information:
1Groupe PiLeJe, 37 Quai de Grenelle, 75015 Paris Cedex 15, Naturopôle, Les Tiolans, 03800 Saint-Bonnet de Rochefort, France.
2IRIMAS, Institut de Recherche en Informatique, Mathématiques, Automatique et Signal, Université de Haute-Alsace, 68093 Mulhouse, France.
3Université Paris Sud, Service d'Hématologie Moléculaire et Cytogénétique Paul Brousse CHU Paris Sud, Inserm UMRS935, 94800 Villejuif, France.
4Service de Génétique Médicale, Groupe Hospitalier de la Région de Mulhouse et Sud-Alsace, 68070 Mulhouse, France.
5Service de Néphrologie, APHP-Hôpital Robert Debré, Paris, France.
6Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut de Chimie de ClermontFerrand, BP 10448, 63000 ClermontFerrand, France.
7Département d'hématologie, Gustave Roussy Cancer Campus, université Paris Saclay, 94808 Villejuif, France.
8Cell Environment, DNA damage R&D, 75020 Paris, France.
Conference/Journal: Biomedicines.
Date published: 2020 Feb 12
Other: Volume ID: 8 , Issue ID: 2 , Special Notes: doi: 10.3390/biomedicines8020031. , Word Count: 194

A link between telomere shortening and oxidative stress was found in aging people and patients with cancer or inflammatory diseases. Extracts of Astragalus spp. are known to stimulate telomerase activity, thereby compensating telomere shortening. We characterized a multi-component hydroethanolic root extract (HRE) of Astragalus mongholicus Bunge and assessed its effects on telomeres compared to those of danazol. Astragalosides I to IV, flavonoids, amino acids and sugars were detected in the HRE. Samples of peripheral blood lymphocytes with short telomeres from 18 healthy donors (mean age 63.5 years; range 3286 years) were exposed to a single dose of 1 µg/mL HRE or danazol for three days. Telomere length and telomerase expression were then measured. Significant elongation of telomeres associated to a less toxicity was observed in lymphocytes from 13/18 donors following HRE treatment (0.54 kb (0.15-2.06 kb)) and in those from 9/18 donors after danazol treatment (0.95 kb (0.06-2.06 kb)). The rate of cells with short telomeres (<3 kb) decreased in lymphocytes from all donors after exposure to either HRE or danazol, telomere elongation being telomerase-dependent. These findings suggest that the HRE could be used for the management of age-related diseases.

KEYWORDS: Astragalus mongholicus Bunge; aging; danazol; telomerase; telomere

PMID: 32059353 DOI: 10.3390/biomedicines8020031