Whole Body Vibration Triggers a Change in the Mutual Shaping State of Intestinal Microbiota and Body's Immunity.

Author: Song N1,2, Liu X1,2, Feng Q3,4,5, Xu M6, Lan X3,4, Li M3,4, Liu R6, Li C7, Dong T8, Wang D1,2, Liu S1,2
1School of Basic Medical Science, Shandong University, Jinan, China.
2Department of Rehabilitation Medicine, Binzhou Medical University Hospital, Binzhou, China.
3Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Department of Human Microbiome, School and Hospital of Stomatology, Shandong University, Jinan, China.
4Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Department of Human Microbiome, School and Hospital of Stomatology, Shandong University, Jinan, China.
5State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
6School of Environmental Science and Engineering, Shandong University, Jinan, China.
7Department of Evidence Identification, Institute of Forensic Science of China, Beijing, China.
8Department of Breast Thyroid Surgery, Shandong Provincial Hospital, Shandong University, Jinan, China.
Conference/Journal: Front Bioeng Biotechnol.
Date published: 2019 Nov 29
Other: Volume ID: 7 , Pages: 377 , Special Notes: doi: 10.3389/fbioe.2019.00377. eCollection 2019. , Word Count: 271

Whole body vibration (WBV) is a non-invasive physical therapy that has recently been included in the hospital's patient rehabilitation training catalog, but its health effects have not been sufficiently studied. In the present study, to examine the possible effects of WBV on immune cell differentiation, the IFN, IL-4,-17, F4/80 and CD3,-4,-8,-11b,-11c,-19 markers were used to characterizing the cells in mouse spleen. The results showed that the CD4 and CD25 positive lymphocytes in the spleen were significantly increased in the WBV group, and the population of Treg cells was enhanced significantly in response to WBV. Since the differentiation in immune cells is usually associated with microbiota, therefore the intestinal flora was characterized in mice and human individuals. The results indicated that WBV significantly reduced the α-diversity of mouse intestinal microbiota. Moreover, the principal coordinate analysis (PCoA) results indicated that the β-diversities of both mice and human fecal microbiota increased after WBV. Analysis of the bacterial composition indicated that the contents of a variety of bacteria changed in mice upon the stimulation of vibration, such as Lactobacillus animalis in mice, and Lactobacillus paraplantarum and Lactobacillus sanfranciscensis in human. The succeeding correlation analysis revealed that some bacteria with significant content variations were correlated to the regulatory T cell differentiation in mice and physical characteristics in human. Our research will provide the basis for future non-invasive treatment of microbial and immune related diseases.

Copyright © 2019 Song, Liu, Feng, Xu, Lan, Li, Liu, Li, Dong, Wang and Liu.

KEYWORDS: Lactobacillus; Treg; correlation; human microbiota; immune cell differentiation; mouse microbiota; physical characteristics; whole body vibration

PMID: 31850333 PMCID: PMC6895539 DOI: 10.3389/fbioe.2019.00377