Osteogenic differentiation of adipose-derived stem cells prompted by low-intensity pulsed ultrasound. 


Author: Yue Y, Yang X, Wei X, Chen J, Fu N, Fu Y, Ba K, Li G, Yao Y, Liang C, Zhang J, Cai X, Wang M.

Affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. 
Conference/Journal: Cell Prolif.
Date published: 2013 Jun
Other:
 Volume ID: 46   ,  Issue ID: 3   ,  Pages: 320-7   ,  Special Notes: doi: 10.1111/cpr.12035.   ,  Word Count: 167  
    

OBJECTIVES:

Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) regulated by LIPUS, and also in a preliminarily manner, we have discussed diverse effects of different duty ratio parameters.

MATERIALS AND METHODS:

Mouse adipose stem cells were isolated and osteogenically induced. Then they were treated with LIPUS for 10 min/day for 3 days, 5 days and 7 days, respectively. Finally, effects of LIPUS on osteogenic differentiation of the ASCs were analysed by real-time PCR, western blotting and immunofluorescence.

RESULTS:

Our data indicated that LIPUS promoted mRNA levels of runt-related transcription factor 2, osteopontin and osterix in the presence of osteo-induction medium; moreover, protein levels of runt-related transcription factor 2 and osteopontin were upregulated.

CONCLUSIONS:

We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.

© 2013 Blackwell Publishing Ltd.

PMID: 23692090 [PubMed - in process]