Method for conducting microarray study of oxidative stress induced gene expression


Author: Subbaram S, Melendez JA, Chittur SV

Affiliation: Center for Immunology & Microbial Disease, Albany College of Medicine, Albany, NY, USA
Conference/Journal: Methods Mol Biol.
Date published: 2009
Other:
  Issue ID: 477   ,  Pages: 357-68   ,  Word Count: 76  
    Cellular systems produce reactive oxygen species during the process of metabolism. Oxidative stress results in the activation or repression of many genes in important signaling pathways. DNA microarrays allow for a high throughput evaluation of the changes in gene expression levels in any biological system. In this study, we describe a method to employ gene expression microarrays to study the transcriptional changes in redox-engineered cell lines that will overexpress MnSOD and/or catalase in the mitochondria.