Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells Author: Takashi Nishio1, Reina Kishi2, Kana Sato2, Kazuomi Sato3 Affiliation: <sup>1</sup> Graduate School of Agriculture, Tamagawa University, 6-1-1 Machida, Tokyo 194-8610, Japan. <sup>2</sup> Department of Life Sciences, College of Agriculture, Tamagawa University, 6-1-1 Machida, Tokyo 194-8610, Japan. <sup>3</sup> Graduate School of Agriculture, Tamagawa University, 6-1-1 Machida, Tokyo 194-8610, Japan; Biosystems &amp; Biofunctions Research Center, Tamagawa University Research Institute, 6-1-1 Machida, Tokyo 194-8610, Japan. Electronic address: kzsato@agr.tamagawa.ac.jp. Conference/Journal: Mutat Res Genet Toxicol Environ Mutagen Date published: 2022 Nov-Dec Other: Volume ID: 883-884 , Pages: 503562 , Special Notes: doi: 10.1016/j.mrgentox.2022.503562. , Word Count: 215 Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells. In this study, cells were irradiated with 2-50 W/m2 blue light (465 nm) for several time duration. Exposure to blue light decreased cell viability, but the pan-caspase inhibitor Z-VAD-FMK rescued blue light-induced cell death. Blue light also inhibited cell proliferation and arrested the cell cycle. Blue light-irradiated cells displayed several apoptotic features, like depolarized mitochondrial membranes and enhanced caspase-3 activity. Furthermore, blue light induced strand breaks in the genomic DNA in a dose- and time-dependent manner but did not induce the formation of cyclobutene pyrimidine dimers. The cell cycle inhibitor p21 and the pro-apoptotic gene Bax were upregulated in blue light-exposed cells, whereas the anti-apoptotic gene Bcl-2 and the apoptosis inhibitor survivin were downregulated. The key enzyme in melanin synthesis, tyrosinase, was upregulated after high-intensity (50 W/m2) blue light exposure and downregulated after low-intensity (0.2 W/m2) blue light exposure. Our study demonstrates that blue light triggers apoptosis and some of its effects are similar to those of ultraviolet radiation. Keywords: Apoptosis; Blue light; Cell cycle; DNA damage; Melanin synthesis; Mitochondria; Tyrosinase; Ultraviolet. PMID: 36462794 DOI: 10.1016/j.mrgentox.2022.503562