Influence of low-intensity pulsed ultrasound on osteogenic tissue regeneration in a periodontal injury model: X-ray image alterations assessed by micro-computed tomography. Author: Wang Y1, Chai Z1, Zhang Y2, Deng F1, Wang Z3, Song J4. Affiliation: 1Chongqing Key Laboratory for Oral Diseases and Biomedical Science, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China. 2Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA. 3Dept. of Biomedical Engineering, Chongqing Medical University, Chongqing, China. 4Chongqing Key Laboratory for Oral Diseases and Biomedical Science, The Affiliated Hospital of Stomatology, Chongqing Medical University, No. 426, North Songshi Road, Chongqing 400015, China. Electronic address: soongjl@163.com. Conference/Journal: Ultrasonics. Date published: 2014 Apr 18 Other: Pages: S0041-624X(14)00088-2 , Special Notes: doi: 10.1016/j.ultras.2014.03.015 , Word Count: 210 OBJECTIVE: This study was conducted to evaluate, with micro-computed tomography, the influence of low-intensity pulsed ultrasound on wound-healing in periodontal tissues. METHODS: Periodontal disease with Class II furcation involvement was surgically produced at the bilateral mandibular premolars in 8 adult male beagle dogs. Twenty-four teeth were randomly assigned among 4 groups (G): G1, periodontal flap surgery; G2, periodontal flap surgery+low-intensity pulsed ultrasound (LIPUS); G3, guided tissue regeneration (GTR) surgery; G4, GTR surgery plus LIPUS. The affected area in the experimental group was exposed to LIPUS. At 6 and 8weeks, the X-ray images of regenerated teeth were referred to micro-CT scanning for 3-D measurement. RESULTS: Bone volume (BV), bone surface (BS), and number of trabeculae (Tb) in G2 and G4 were higher than in G1 and G3 (p<0.05). BV, BS, and Tb.N of the GTR+LIPUS group were higher than in the GTR group. BV, BS, and Tb.N of the LIPUS group were higher than in the periodontal flap surgery group. CONCLUSION: LIPUS irradiation increased the number, volume, and area of new alveolar bone trabeculae. LIPUS has the potential to promote the repair of periodontal tissue, and may work effectively if combined with GTR. Copyright © 2014 Elsevier B.V. All rights reserved. KEYWORDS: Low-intensity pulsed ultrasound, Micro-computed tomography, Periodontal disease PMID: 24815538