Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells. 


Author: Fu N, Yang X, Ba K, Fu Y, Wei X, Yue Y, Li G, Yao Y, Chen J, Cai X, Liang C, Ge Y, Lin Y.

Affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, 610041, China. 
Conference/Journal: Cell Prolif.
Date published: 2013 Jun
Other:
 Volume ID: 46   ,  Issue ID: 3   ,  Pages: 312-9   ,  Special Notes: doi: 10.1111/cpr.12031   ,  Word Count: 111  
    

OBJECTIVE:

The aim of this study was to investigate effects of low-intensity pulsed ultrasound (LIPUS) on differentiation of adipose-derived stem cells (ASCs), in vitro.

MATERIALS AND METHODS:

Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR-γ1, and APN, was examined by real-time PCR. Immunofluorescence (IF) staining was performed to test for PPAR-γ at the protein level.

RESULTS:

Our data revealed that specific patterns of LIPUS up-regulated levels of both PPAR-γ1 and APN mRNA, and PPAR-γ protein.

CONCLUSIONS:

In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.

© 2013 Blackwell Publishing Ltd.

PMID: 23692089 [PubMed - in process]